Welcome back to Genome Editing and CRISPR Cas system playlist in this pdf, we will continue our explanation for CRISPR Cas9 and will discover together: -cytosine base editing -Adenine base editing -prime editing -CRISPR/Cas13 RNA targeting (RESCUE & REPAIR) systems -mitochondrial and chloroplast base editing using TALE

1. Base Editing
"From Single Nucleotide
Polymorphisms to Disease
Correction"

3. Genome Base Editing (BE)
• It’s a technique that allows for precise changes to individual
nucleotides in the DNA sequence without creating DSBs
• It converts one base pair into another without requiring DNA
cleavage
• It’s a new technology for precise modification of DNA or RNA of
living cells at the single base resolution

4. BE
2 Classes of DNA Base
Editors
CBEs
“Cytosine Base Editors”
ABEs
“Adenine Base Editors”

5. Cytosine base editing (CBE)
Cytidine
deaminase
nCas9 UGI

8. Adenine base editing (ABE)
Heterodimeric
deaminase
nCas9

11. Dual base editor
Adenine
deaminase
nCas9
Cytidine
deaminase
UGI

14. Prime Editing (PE)
nCas9
Engineered
Reverse
Transcriptase (RT)

19. CRISPR /Cas13 RNA targeting
systems

20. NA
Diting for
programmable
to
eplacemet

21. NA
Diting for
programmable
to
eplacemet

22. CRISPR /Cas13 RNA targeting
systems

23. NA
diting for
pecific
to
xchange

24. NA
diting for
pecific
to
xchange

25. Organelle editing-based

26. Organelle editing-based
DddA UGI
TALE
array

27. DddA cytidine
deaminase (DdCBE), a
UGI to catalyse
cytosine
deamination, inducing
C-to-T conversions and
mitochondrial targeting
signal (MTS) for editing
mitochondrial DNA

28. Chloroplast transit
peptide (CTP) for
editing Chloroplast
DNA