DNA

1. Quiz Bee
DNA Replication, Protein Synthesis and
Genetic Engineering

2. Mechanics
A
The Quiz bee.
B
The questions are based on DNA
Replication, Protein Synthesis
and Genetic Engineering.
C
Winners will be
determined by the
teacher.
› The class will be divided into
8 groups
› There will be easy, average
and difficult round
› Easy round = 1 point
› Average round = 2 points
› Difficult round = 5 points
› The questions will be flashed
on the screen and the
teacher will read the question
twice.
› There will be a time limit for
each question. The group can
start answering as soon as
the question appears on the
screen.
› The group must show their
answer after the signal is
given by the teacher.
› Easy round has 10 questions.
› Average round has 10
questions.
› Difficult round has 3
questions.
› A total of 45 points.
› The group with the most
accumulated points will be
the winner

4. Easy Round
Multiple Choice

5. Question #1
DNA is created according to the semiconservative model of replication. This
means
A.The replicated DNA contains half original and half new DNA
B.The replicated DNA contains a combination of paternal and maternal
DNA
C.one strand contains all A and T nucleotides, and the other strand
contains all C and G nucleotides
D.The replicated strands are antiparallel

6. Question #1
DNA is created according to the semiconservative model of replication. This
means
A.The replicated DNA contains half original and half new DNA
B.The replicated DNA contains a combination of paternal and
maternal DNA
C.one strand contains all A and T nucleotides, and the other
strand contains all C and G nucleotides
D.The replicated strands are antiparallel

7. Question #2
There are ___ hydrogen bonds between A and T; there are ___ hydrogen
bonds between C and G.
A.2;2
B.1;4
C.2;3
D.3;2

8. Question #2
There are ___ hydrogen bonds between A and T; there are ___ hydrogen
bonds between C and G.
A.2;2
B.1;4
C.2;3
D.3;2

9. Question #3
A biochemist isolates, purifies, and combines in a test tube a variety of molecules needed for DNA
replication. When she adds some DNA to the mixture, replication occurs, but each DNA molecule
consists of a normal strand paired with numerous segments of DNA a few hundred nucleotides long.
What has she probably left out of the mixture?
A.Nucleotides
B.DNA ligase
C.DNA polymerase
D.Okazaki fragments

10. Question #3
A biochemist isolates, purifies, and combines in a test tube a variety of molecules needed for DNA
replication. When she adds some DNA to the mixture, replication occurs, but each DNA molecule
consists of a normal strand paired with numerous segments of DNA a few hundred nucleotides long.
What has she probably left out of the mixture?
A.Nucleotides
B.DNA ligase
C.DNA polymerase
D.Okazaki fragments

11. Question #4
What is the basis for the difference in how the leading and lagging strands of
DNA molecules are synthesized?
A.Helicases and single-strand binding proteins work at the 5' end.
B.DNA ligase works only in the 3' → 5' direction.
C.The origins of replication occur only at the 5' end.
D.DNA polymerase can join new nucleotides only to the 3' end of a
growing strand.

12. Question #4
What is the basis for the difference in how the leading and lagging strands of
DNA molecules are synthesized?
A. Helicases and single-strand binding proteins work at the 5'
end.
B. DNA ligase works only in the 3' → 5' direction.
C.The origins of replication occur only at the 5' end.
D.DNA polymerase can join new nucleotides only to the 3' end of
a growing strand.

13. Question #5
What is the function of mRNA in protein synthesis?
A. To regulate gene expression
B. To replicate DNA
C.To carry the genetic code from DNA to the ribosomes
D.To assemble amino acids into a polypeptide chain

14. Question #5
What is the function of mRNA in protein synthesis?
A. To regulate gene expression
B. To replicate DNA
C.To carry the genetic code from DNA to the ribosomes
D.To assemble amino acids into a polypeptide chain

15. Question #6
Which molecule carries the amino acids to the ribosomes during protein
synthesis?
A. rRNA
B. DNA
C.mRNA
D.tRNA

16. Question #6
Which molecule carries the amino acids to the ribosomes during protein
synthesis?
A. rRNA
B. DNA
C.mRNA
D.tRNA

17. Question #7
What occurs during the elongation phase of translation?
A. mRNA binds to the small ribosomal subunit
B. Polypeptide chain is synthesized
C.Release factor recognizes the stop codon
D.mRNA is transcribed from DNA

18. Question #7
What occurs during the elongation phase of translation?
A. mRNA binds to the small ribosomal subunit
B. Polypeptide chain is synthesized
C.Release factor recognizes the stop codon
D.mRNA is transcribed from DNA

19. Question #8
What is recombinant DNA technology?
A. The process of transferring genes from one organism to another
B. The process of editing genes within an organism's DNA
C. The process of selecting and breeding organisms for desired traits
D. The process of analyzing an organism's genetic material

20. Question #8
What is recombinant DNA technology?
A. The process of transferring genes from one organism to another
B. The process of editing genes within an organism's DNA
C. The process of selecting and breeding organisms for desired traits
D. The process of analyzing an organism's genetic material

21. Question #9
What is the role of restriction enzymes in genetic engineering?
A. To amplify DNA segments through PCR
B. To cut DNA at specific sites and create recombinant DNA molecules
C.To analyze the sequence of DNA using gel electrophoresis
D.To introduce foreign DNA into host cells

22. Question #9
What is the role of restriction enzymes in genetic engineering?
A. To amplify DNA segments through PCR
B. To cut DNA at specific sites and create recombinant DNA molecules
C.To analyze the sequence of DNA using gel electrophoresis
D.To introduce foreign DNA into host cells

23. Question #10
What is the significance of genetically modified organisms (GMOs)?
A. They can have improved nutritional content or increased resistance to pests or
diseases
B. They can contribute to sustainable agriculture and food security
C.They can be used in the production of medicines and biofuels
D.All of the above

24. Question #10
What is the significance of genetically modified organisms (GMOs)?
A. They can have improved nutritional content or increased resistance to pests or
diseases
B. They can contribute to sustainable agriculture and food security
C.They can be used in the production of medicines and biofuels
D.All of the above

25. Average Round
Identification

26. QUESTION # 1
Form of RNA that carries genetic
information from the nucleus to the
cytoplasm, where it serves as a
template for protein synthesis
messenger
RNA (mRNA)

27. QUESTION # 2
Process by which mRNA is decoded
and a protein is produced.
Translation

28. QUESTION # 3
Group of three bases on a tRNA
molecule that are complementary to
an mRNA codon
Anticodon

29. QUESTION # 4
A self-replicating material present in
nearly all living organisms as the
main constituent of chromosomes. It
is the carrier of genetic information.
DNA

30. QUESTION # 5
A segment of DNA on a chromosome
that codes for a specific trait
Genes

31. QUESTION # 6
The process by which information
encoded in DNA directs the synthesis
of proteins or, in some cases, RNAs
that are not translated into proteins
and instead function as RNAs.
Gene
Expression

32. QUESTION # 7
The most common genetically
modified organism where gene of
interest is inserted
Bacteria (E.
Coli)

33. QUESTION # 8
A specific nucleotide sequence in
DNA that binds RNA polymerase and
indicates where to start transcribing
RNA.
Promoter

34. QUESTION # 9
The first step of gene expression, in
which a particular segment of DNA is
copied into mRNA
Transcriptio
n

35. QUESTION # 10
A process of removing non-coding
sequence of mRNA at the particular
stage of protein synthesis.
Intron
Splicing

36. Difficult Round
Problem Solving

37. Question #1
What is the correct sequence of the basic steps in genetic engineering?
I. Selection of vector and insertion of a gene
II. Isolation of desired gene
III. Expression of cloned genes
IV. Identification, isolation of recombinant gene cells
V. Transfer of DNA vector into host cells
II, I, V, IV, III

38. Question #2
What is the sequence of
protein after translation
process?

39. Question #3 Place the event in the correct order
V, II, III, I, IV

42. ADD A SLIDE
TITLE - 4