15 pages slides of paper Chromatography And process Principle Types Instrumentation

1. HEMCHAND YADAW UNIVERSITY,
DURG
Pt. Devi Prasad Chaubey Govt. College,
Saja

2. PAPER CHROMATOGRAPHY
SHRISHTI RAJPUT

3. • Introduction
• Historical development
• Types of chromatography
• Paper chromatography
• Principal
• Instrumentation
• Methedology
• Modes of paper
chromatography
• Application
• Recent research

4. Introduction
Paper Chromatography is defined as a procedure by
which solute are separated dynamic differential
migration process in a system consisting of two or
more face one of which moves continuously in a
given direction
Two phases are
• Stationary phase
• Mobile phase

5. •In 1906 chromatography was first
discovered by M. TSWETT.
•In 1942 partition chromatography
was developed by MARTIN AND
SYNG.
•In 1961 paper chromatography was
discovered by SEHON BEIN.
Historical development

6. Types of chrometography

7. Paper Chromatography
•Paper chromatography is defined as technique in
which the analysis of unknown substance is carried
out mainly by the flow of solvent on specially
designed filter paper.
•In 1961 paper Chromatography was developed by
SEHON BEIN.

8. Principle
The principle of separation is mainly partition rather than
absorption cellulose layers in filter paper contains
moisture which acts as stationery phase. Organic solvents
or buffers are used as mobile phase. Instead of water as
stationery phase other organic solvent can be used by
suitable modification.

9. Instrumentation

10. Methodology
• Obtain a chromatogram jar, a piece of fresh spinach, and
a length of chromatography paper
• Cut the tip of the paper such that it forms a point of a
triangle
• Draws a line across the paper 1cm up from the triangle
this is start line.
• Using a capillary tube transfer a drop of the green
pigment sample to the centre of start line.

11. • Ascending chrometography
• Descending chromatography
Modes of paper chrometography

12. Application
•Paper Chromatography is specially used for
separation of mixture having polar and non polar
compound
• For separation of amino acid
• It is used to determine organic compound
biochemical in urine etc.
• Sometime used for evolution inorganic compound
like salt and complex

13. Recent research
• Denis. V, Bochko. V, Sergey. V, Sysolyatin Alexander. I, Kalashnikov,
Irina. A, Surmacheva
• 20 April 2011
• Shikimic Acid: Review of it's analytical, isolation, and purification
techniques from plants and microbial sources.
• Institute for problem of chemical and energetic technology's Siberian
branch of the Russians academy of sciences.

14. THANK YOU