Leukopoiesis study of leukocytes. In this we will study about all about WBC. Iqra Rubab student of bs Mlt 5 semester in sarhad university of science and technology Peshawar.

1. Leukopoiesis
Submitted by
Iqra Rubab

2. Types of White Blood cells

3. LEUKOPOIESIS
Leukopoieses is form of Hematopoiesis
in which white blood cells or
leukocytes are formed in bone marrow
located in bones in adults and
hematopoietic organs in fetus
Granulocytopoiesis
Agranulocytopoieses

4. • All leukocytes orignate from
Hemocyto blasts
Myeloid stem cells
• Myeloblasts
• Monoblasts
Lymphoid stem cells
• Lymphoblasts

5. BONE MARROW;THE SITE OF
LEUKOPOIESES
Vascular
compartments:
Extensive network of sinusoids
Hematopoietic
compartments:
Islands of hematopoietic cells (Hematopoietic
cells in various stages of maturation)

6. • These are responsible for stimulation or inhibition of
production, differentiation of mature blood cells and
their precursor
• Hematopoietic proginator cells require cytokines for
their growth and survival and prevent cells from dying
by inhibiting apoptosis
• Cytokines include interleukins ILS, lymphokines,
monokines, interferons, chemokines and
colonystimulating factor CFUs
Cytokines and Growth Factors

7. • Colony-Stimulating factors [CFUs]
These are produced by different cells. They have high specificity for their target
cells and activated at low temperature
The names of the individual factor indicate predominant cell lines
• Interleukins
Mediate multiple, highly complex communications between various classes of
WBCs
Originally were named according to their specific function, such as
lymphocyte-activating factors
Hormonal Control of Leukopoieses

8. Large cells with large nucleus. This stage exists for all granulocytes
During this stage (azurophillic) granules are formed
The developing neutrophils can now be differentiate from basophils and eosinophils as neutrophils
specific granules are now being formed
At this .stage nucleus elongate, becomes heterochromatic and has a kidney like shape mitosis can no
longer occur. Differentiation is no much clearer and far greater number then primary granules
Neutrphils
Myoblast
Promylocytee
Neutrophilic Myelocyte
Neutrophilic Metamyelocyte

9. Nucleus elongate further and represents a
horse shoe. Nucleus starts to segment
Mature neutrophils are formed and the nucleus is
segmented and has 3 to 5 lobes. This lobular structure
of the nucleus gives rise to the name
polymorphonuclear neutrophils
Band cells
Neutrophils

10. These stages are common to all granulocytes and no distinction can
be made between different cell lines
Specific granules start to appear in myelocyte and once the cell
has reached the metamyelocyte stage it cannot undergo further mitosis
Nucleus elongates further and represents a horse shoe
. Nucleus starts to segment
Mature cell has a bi-lobed nucleus. There are species
specific variation in granular size once stained
Eosinophils
Myeloblast and Promyelocyte
Eosinophilic Myelocyte and Metamyelocyte
Band cells
Eosinophils

11. BASOPHILS
Have round lobulated nucleus with condensed chromatin.
Nucleoli may or may not apparent. Cytoplasm contain large
blue- black secondary granules. Primary granules may or may
not be seen
Mature Basophils:
Contain lobulated nucleus with granules. The chromatin
pattern,if available,is clumped. Cytoplasm is colourless contain
blue-black granules
Basophils
Immature Basophills
Mature Basophills

12. This is the first stage after cell has differentiated into the CFU-M
Cell has large nucleus and consist of tow populations:
One rapidly dividing and other slowly dividing
These cells have kidney shaped nucleus and cytoplasm is often vacuolated.
Once monocyte enters tissue it differentiate into macrophage
Monocyte
Monoblast
Pronomocyte
Monocyte
Macrophage

13. • Lymphoblast:
Lymphocytes are activated by antigen nucleus and cytoplasm growth
increased in volume as well as mRNA and proteins synthesize
Smaller than lymphoblasts and larger than mature lymphocytes
Lymphocytes are mature, infection fighting cells
 B-lymphocytes
 T-lymphocytes
Lymphocytes
Lymphoblast
Prolymphocyte
Lymphocyte

14. Leukocyte Disorders

15. • Leukopoieses is formation of WBCs, occurs in Bone
marrow
• Granulocytes and monocytes derived from Myeloid stem
cells, whereas lymphocytes from Lymphoid stem cells
• Leukopoieses involved series of mitosis and
differentiation
• Leukopoieses are regulated by several growth factors
Conclusion

16. • https://www.nature.com/subjects/leukopoieses
• https://en.wikivet.net/Leukopoieses
• https://www.ncbi.nlm.nih.gov/mesh
• https://connects.catalyst.havard.edu/Profiles//display/Leukopoises
Refrences