This presentation will give you overall information about the application in Proteomic Science and Immunotherapeutics. It covers Proteomics, Genomics, Metabolomics, Functionomics, Nutrigenomics and Types of Immunotherapeutics, Humanized Antibody Therapy, Immunotherapeutics in Clinical Practice.

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Dr. Rajendra Gode Institute of Pharmacy, Amravati
“ Application of Proteomic Science
&
Immunotherapeutics”
Presented by
Shraddha S. Raut
M. Pharm (Pharmacology)
Guided by
Prof. Shubhangi Bhagat
M. Pharm (Pharmacology)

2.  Contents
Introduction
Genomics
Proteomics
Metabolomics
Functionomics
Nutrigenomics
Immunotherapeutics
Types of Immunotherapeutics
Humanized Antibody therapy
Immunotherapeutics in clinical practice
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3.  Introduction
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• Proteomic tools
Proteomic tools are methods used to study the structure, function and interactions of
proteins in biological system.
• Importance of Proteomic Tools
1. To identify and validate targets for drug discovery
2. To monitor drug responses
3. To personalize therapies

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Genome : It is a complete set of gene in an organism.
Gene + Non-coding DNA + Genetic material of mitochondria
Genomics
It is an interdisciplinary branch of science which deals
with study of structure, comparison, function and evaluation of
genome.
• It studies the expression of genes to produce proteins along
with sequencing and analysis of genome.
Genomics

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Types of Genomics
Functional
genomics
Structural
genomics
Comparative
genomics
Epigenomics Metagenomics
• Application of Genomics
Biotechnology
Conservation of
species

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Genomic medicine

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Proteome : It is a complete set of protein in an organism.
• It is derived from genome by gene expression.
• It differs from cell-to-cell and time-to-time.
Proteomics
It involves identification and analysis of total protein of an
individual.
It also provides direct measure of quantity present.
Proteomics

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Types of Proteomics

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• Application of Proteomics
1) To understand structure and function of different proteins
2) To understand protein-protein interaction
3) To detect defect in protein structure, function or expression pattern
4) Useful in identifying biomarkers that may be used to diagnose disease
5) Plays important role in drug development process

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Metabolome : All metabolites of cell, tissue, organ or organism.
Metabolomics
It is a scientific study of metabolome or chemical
processes involving metabolites.
• It involves study of biological fluid to analyze changes in
metabolite and corelate with disease development.
• GC-MS and NMR spectroscopy is used.
Metabolomics

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Types of metabolites
In Human In Plants
• Endogenous metabolites
• Exogenous metabolites
• Primary metabolites
• Secondary metabolites
• Application of Metabolomics
1) Disease diagnosis
2) Toxicity assessment
3) In functional genomics
4) Environmental metabolomics

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• It is a field that deals with creating a unique profile
of vital functions of an individual.
• It is also called as “Fingerprinting of vital functions
of an individual.”
Unique profile
used to predict the future aspects of health, disease and
lifespan of an individual
Functionomics

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• Application of Functionomics
1) To understand function of genes
2) To measure molecular activities
3) To study the arterial function, fitness, metabolism and ANS of the person under
investigation and then compare it with normal person of same age and gender.

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Nutriome : Ideal combination of micronutrients and their doses that promotes stability to
genome and DNA.
Nutrigenomics
• It is a branch of genomics which studies the influence of
genetic variation on nutrition
nutrients on gene
• It deals with correlation of gene expression with absorption, metabolism and elimination
of nutrients.
• It gives the concept of “personalized nutrition.”
Nutrigenomics

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• Examples of Nutrigenomics
1) Lactose intolerance
2) Phenylketonuria
3) Caffeine

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• Application of Nutrigenomics
1) Obesity - Used to take preventive measures to treat and manage obesity.
2) Cancer - Prevent DNA damage and cancer development
3) Anti-ageing - Prevent premature ageing of cell
4) Used for prevention of disease
5) It develops neuroprotective mechanism
6) It helps to classify genes that trigger diet-related disease

17.  Immunotherapeutics
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Agents that are used to either activate or suppress the immune response are
called as Immunotherapeutics.
• And therapy is termed as Immunotherapy which works by 3 different mechanism
1. Non-specific activation of body’s immune system
2. Specific activation of immune cells
3. Supplementation of immune system with immune products

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 Types of Immunotherapeutics
Monoclonal antibodies
Fusion protein
Recombinant Cytokine
Soluble Cytokine receptor
Cellular therapies
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1) Monoclonal antibodies
• It bound to same epitope of an antigen.
• These are produced by immune cells which are clones of unique parent cell by
Hybridoma technology.
• Types :
Murine antibody
Chimeric antibody
Humanized antibody
Fully Humanized antibody

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2) Fusion Proteins
• Two or more genes encoding for different proteins are fused to form fusion
protein .
• Also called as chimeric or hybrid protein.
• These are made by rDNA technology.
• Purpose : To incorporate desirable properties of two or more proteins in a
single protein molecule.
• Examples : Etanercept
Aflibercept

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3) Recombinant Cytokines
• Cytokines are small proteins which plays key role in cell communication and
cell signaling.
• Cytokines includes Chemokines, Interferons, Interleukins and Lymphokines
which are released by immune cells.
• Theses are made by rDNA technology and use to stimulate immune response.
• Example : Interferons
Interleukins

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4) Soluble Cytokine receptors
• These agents bind to circulating cytokines and prevent its binding to their actual
receptors located on immune cells and prevent activation of immune system.
• These are made by rDNA technology.
• Ex : Anakinra
5) Cellular therapy
• In this immune cells are isolated (Leukapheresis) and then activated by incubating
with antigen and colony stimulating factor.
• The activated immune cells are returned to human to attack on antigen.
• Ex : Sipuleucel-T

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 Humanization Antibody Therapy
• Antibody : Protein used by immune system to identify and neutralize foreign objects.

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Types of Immunoglobulin

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Humanization of antibody therapy
• It is the process of removing mice antigenic portion from monoclonal antibody and
replacing with human variants is called as humanization of antibody.
• Such antibodies are called as Humanized antibody.
• Monoclonal antibodies are produced by one type of parent cell using Hybridoma
technology.
• Humanized antibodies are produced by using rDNA technology.

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Examples of Humanized antibodies with their Uses

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 Immunotherapeutics in Clinical practices
1. Immunotherapy in Cancer treatment
• Identifying targets
Scientists are discovering new targets on cancer cells that can be exploited by
immunotherapy, leading to more effective treatment options.
• Combination therapies
Combining different types of immunotherapies with other treatments like chemotherapy
or radiation can lead to synergistic effects, improving outcomes for patients.
• Increasing survival rates
Immunotherapeutics have shown promising results in improving long-term survival rates
in various types of cancer, offering new hope and extended quality of life for patients.

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2. Immunotherapy in Autoimmune disorder
• Immunotherapy is not only transforming cancer
treatment but also providing novel approaches to
manage autoimmune diseases, such as rheumatoid
arthritis, multiple sclerosis, and lupus.
• By modulating the immune response,
immunotherapies can help reduce disease symptoms
and improve patients' quality of life.

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3. Immunostimulant
• To boost immune system
Ex. Bacillus Calmette Guerin
• To stimulate bone marrow to make WBCs for treatment of neutropenia.
Ex. Sargramostin
3. Immunosuppressant
• Organ transplantation
• Selective immunosuppression
• Severe inflammatory bowel diseases

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 References
1) A Text Book of Cellular and Molecular Pharmacology by Dr. Sahu and Dr.
Tiwari,1st edition, Nirali Prakashan, Pg no. 4.9 - 4.14
2) Essentials of Medical Pharmacology, 8th Edition by KD Tripathi, Pg no. 937-945

32. T H A N K
Y O U
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Any questions