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Magnification and microscopes
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- 2. Mutual dependence: Science& Technology Technology…the pragmatist Does it work? Is it useful? Is it sustainable? Science…the idealist What is it? How does it work? What does it tell us? Regents Biology
- 3. Mutual dependence: Science& Technology Microscopes Electron microscopes Higher resolution Dead samples Expensive Optical (light) microscopes Lower resolution Live samples Less expensive Regents Biology
- 4. Micrographs Scanning electronmicrograph (SEM) of various Pollen. Public domain image reference: Dartmouth Electron Microscope Facility, Dartmouth College Regents Biology
- 5. Size matters RegentsBiology micron = micrometer = 1/1,000,000 meter diameter of human hair = ~20 microns Miss N, Show them the interactive!!
- 6. Microscopic units ofmeasurement Regents Biology
- 7. 1 metre (m)= ________ millimetres (mm) 1 mm = ________ microns (μm) 1 μm = ________ nanometres (nm) 1 mm = ________ nm 1 m = ________ μm 1 m =________ nm Regents Biology
- 8. 1 m =1,000 mm = 103mm 1 mm = 1,000 μm= 103μm 1 μm = 1,000 nm= 103nm 1 mm = 1,000,000 nm = 106nm 1 m = 1,000,000 μm = 106μm 1 m = 1,000,000,000nm =109 nm Regents Biology
- 9. 10-3 m =______ m = _____mm 10-6 m = ______ m = _____mm 10-9 m = ______ m = _____mm Regents Biology
- 10. 10-3 m =0.001m = 1 mm 10-6 m = 0.000001 = 1 μm 10-9 m = 0.000000001= 1nm Regents Biology
- 11. Electron microscope range: _____ to _____ Light microscope range: _____ to _____ Unaided human eye range: _____ to _____ Unaided? Regents Biology
- 12. Electron microscope range: 1 nm to 100 μm Light microscope range: 1 μm to 1 mm Unaided human eye range: 1 mm upwards Regents Biology
- 13. Name that micrograph Light microscope or Electron microscope? Regents Biology
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- 15. Unstained Regents Biology19 Light microscope
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- 17. Regents Biology 20Light microscope
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- 20. Regents Biology 22Light microscope
- 21. Regents Biology 23 Tick
- 22. Tick Regents Biology23 Electron microscope
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- 24. Regents Biology 24Electron microscope
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- 26. Regents Biology 25Light microscope
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- 28. Regents Biology 26Electron microscope
- 29. Regents Biology 27 Aphid
- 30. Aphid Regents Biology27 Electron microscope
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- 32. Regents Biology 28Light microscope
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- 34. Regents Biology 29Light microscope
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- 36. Regents Biology 30Electron microscope
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- 38. Regents Biology 31Electron microscope
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- 40. Regents Biology 32Electron microscope
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- 42. Regents Biology 33Electron microscope
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- 44. Regents Biology 35Light microscope
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- 46. Regents Biology 36Light microscope
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- 48. Regents Biology 37Light microscope
- 49. THE END! RegentsBiology
- 50. Magnification Magnification =ocular lens x objective lens A microscope has an eyepiece lens with a power of 20X. The objective lens being used has a power of 10X. What is the total magnification? Regents Biology
- 51. Using Scale Bars Using a scale bar: The image at right is of a virus-like particle. The bar represents 50nm. It is observed under x200. Calculate the actual size of the image. Regents Biology 50nm
- 52. Using a scalebar: Image is 50nm in diameter Regents Biology 50nm Using Scale Bars
- 53. A specimen is1cm in diameter under a power of x600. What is the actual size of the specimen? Regents Biology
- 54. Magnification: I AM Regents Biology Image size Actual size Magnification Watch yo units!! Measure with ruler Need to figure this out Given to us
- 55. 1. Measure thesize of the image. …..1cm (10mm) 2. Find the actual size of the sample I/AM I=10mm M=600 A=? A=10mm/600 = 0.0167mm =16.67 μm Regents Biology
- 56. Your Turn! RegentsBiology
Editor's Notes
- #2 Lesson 5-7 Practical 1
- #3 Technology and science are often used interchangably, but this requires some clarification. Technology emerged before science. Materials were used to produce and used long before there was an understanding that different materials had different properties that could be used for different purposes. Modern science often is the opposite. Scientific understanding is often the basis for technological development. These developments in technology in turn drive developments in science. So technology applies the question: “does it work?” to its pursuit. Science is less bothered by the knowledge for the sake of knowledge Desprite their mutual dependence, they are based on different values. Science: evidence, rationality and the quest for deeper understanding Tech: practical, appropriare and useful and an increasing emphasis on sustainability
- #4 Technology and science are often used interchangably, but this requires some clarification. Technology emerged before science. Materials were used to produce and used long before there was an understanding that different materials had different properties that could be used for different purposes. Modern science often is the opposite. Scientific understanding is often the basis for technological development. These developments in technology in turn drive developments in science. Both electron and light microscopes are technical devices which are used for visualizing structures that are too small to see with the unaided eye, and both types have relevant areas of applications in biology and the materials sciences. And this is pretty much it. The method of visualizing the structures is very different. Electron Microscopes use electrons and not photons (light rays) for visualization. The first electron microscope was constructed in 1931, compared to optical microscopes they are a very recent invention.
- #6 http://learn.genetics.utah.edu/content/cells/scale/
- #13 Anything below 1 micron requires an electron microscope. But there are STILL things we can’t see clearly with EM, like cell membranes!
- #57 Calculation and drawing practice and homework Lesson 7 Use microscopes to observe samples. Practical 1.