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This is where my journey started, at 
Rodale’s FST in the Lehigh Valley
40% of the world’s population and 
55% of children under the age of 5 
live in the tropics. We need to 
demonstrate that regenerative 
agricultural practices work in the 
tropics.
The Carbon Underground Tropical 
Farming Systems Trial 
Trial Design
Same cassava starter cuttings, 
same farmers, same sun, same 
starter soil, same water
Then a drought happened….
The organic cassava
The “conventional” cassava
Marked Failure to Germinate in 
Conventional Fields 
2500 
2000 
1500 
1000 
500 
0 
BD.A BD.B OR.A OR.B CV.A CV.B 
Average number plants per treatment group 
Average number per treatment group that did 
not germinate
40.0000 
35.0000 
30.0000 
25.0000 
20.0000 
15.0000 
10.0000 
5.0000 
0.0000 
Average % of plants that did not germinate per treatment group 
BD.A BD.B OR.A OR.B CV.A CV.B 
Average % did not germinate per treatment 
replication set
We then replicated germination 
conditions in a more controlled 
environment, taking soil from each of 
the six farming systems and replicating 
the drought conditions by planting 
under greenhouse plastic.
We found that the germination 
results under greenhouse plastic 
were the same as in the field. Here 
are two photos to show the 
differences between regenerative 
organic and conventional 
treatments.
On the left, conventional. On the 
right, regenerative organic.
Seeking explanations for the 
organic success vs. the 
conventional failure
Bacterial Dynamics 
14000 
12000 
10000 
8000 
6000 
4000 
2000 
0 
OR A OR B BD A BD B CV A CV B 
Average Bacteria Biomass (ug/g) Feb. 2014 
Average Bacteria Biomass (ug/g) Apr. 2014
Protozoan Dynamics 
100000 
90000 
80000 
70000 
60000 
50000 
40000 
30000 
20000 
10000 
0 
OR A OR B BD A BD B CV A CV B 
Average Protozan Population Feb. 2014 
Average Protozan Population Apr. 2014
Could there be a deeper cause? 
Recall that soil organic carbon can 
retain up to 40X its weight in 
water….
So we dug down for information. 
More precisely, we took 810 soil 
samples down to 80 cm, with 135 
samples per farming system
And a note on determining carbon 
sequestration: we use the The 
Earth Partners’ soil carbon 
methodology, approved as a 
Verified Carbon Standard
And now we have 9 months of data. 
Remember: the organic farming 
systems all used compost tea, 
compost mulch (applied at a rate of 
approximately 7 tonnes per hectare, 
of which approximately 2 tonnes is 
organic C), and only had an initial 
tilling. The conventional systems 
used herbicides, pesticides, and had 
two tillings.
As you’ll see, after the initial 
tilling, where a decades-old weed 
mat was ploughed under, we 
introduced a significant amount of 
“labile” or unstable carbon over 
baseline.
Let’s look at differing soil depths 
to see if we can find out what’s 
happening with soil organic 
carbon
COA COB BDA BDB ORA ORB 
TREATMENT 
0-10 kg of C per m² Pre-till 
0-10 kg of C per m² Post-till 
0-10 kg of C per m² 12 de agosto 
78.50 
67.29 
56.07 
44.85 
33.63 
kg of C per m² 
43.77 44.67 
42.61 
46.54 
41.23 
48.53 
Change in Kg of Carbon per m² at 0-10 cm 
SOURCE: RAW DATA, TFST TEAM 
SEPT, 2014 
P VALUE = NOT STATISCALLY SIGNIFICANT
COA COB BDA BDB ORA ORB 
TREATMENT 
10-20kg of C per m² Pre-till 
10-20 kg of C per Post-till 
10-20 kg of C per m² 12 de agosto 
57.70 
49.80 
41.90 
34.00 
26.10 
kg of C per m² 
32.57 32.72 
36.42 36.95 
33.57 
36.28 
Change in Kg of Carbon per m² at 10-20 cm 
SOURCE: RAW DATA, TFST TEAM 
SEPT, 2014 
P VALUE = NOT STATISCALLY SIGNIFICANT
COA COB BDA BDB ORA ORB 
TREATMENT 
20-40 kg of C per m² Pre-till 
20-40 kg of C per m² Post-till 
20-40 kg of C per m² 12 de agosto 
68.26 
59.09 
49.91 
40.73 
31.55 
kg of carbon per m² 
47.83 47.42 
48.84 
47.42 
44.75 
50.12 
Change in Kg of Carbon per m² at 20-40 cm 
SOURCE: RAW DATA, TFST TEAM 
SEPT, 2014 
P VALUE = NOT STATISCALLY SIGNIFICANT
COA COB BDA BDB ORA ORB 
TREATMENT 
40-60 kg of C per m² Pre-till 
40-60 kg of C per m² Post-till 
40-60 kg of C per m² 12 de agosto 
54.60 
45.40 
36.21 
27.02 
17.83 
kg of carbon per m² 
28.16 
30.44 
35.13 
33.63 
38.52 
41.13 
Change in Kg of Carbon per m² at 40-60 cm 
SOURCE: RAW DATA, TFST TEAM 
SEPT, 2014 
P VALUE = NOT STATISCALLY SIGNIFICANT
COA COB BDA BDB ORA ORB 
TREATMENT 
60-80 kg of C per m² Pre-till 
60-80 kg of C per m² Post-till 
60-80 kg of C per m² 12 de agosto 
50.45 
39.40 
28.35 
17.30 
6.25 
kg of carbon per m² 
25.90 
23.16 
25.64 27.15 28.08 
38.12 
Change in Kg of Carbon per m² at 60-80 cm 
SOURCE: RAW DATA, TFST TEAM 
SEPT, 2014 
P VALUE = NOT STATISCALLY SIGNIFICANT
So let’s focus on the 
trends at the 60-80 cm 
depth
MEDIAN LABILE CARBON ABOVE BASELINE AFTER THE TILLAGE 
AT A 60-80 CM DEPTH 
Test:LSD Fisher Alfa=0.05 DMS=11.51007 
Error: 41.8608 gl: 12 
Treatment Median n E.E. 
Conv A 13.88 3 3.74 A 
Conv B 9.85 3 3.74 A B 
ORG B 8.34 3 3.74 A B 
ORG A 4.37 3 3.74 A B 
BD B 1.74 3 3.74 B 
BD A 0.65 3 3.74 B 
Medians that share the same letter are not statistically significant (p > 0.05) 
SOURCE: RAW DATA, TFST TEAM 
SEPT, 2014 
P VALUE = NOT STATISCALLY SIGNIFICANT
MEDIAN CHANGE COMPARED TO POST TILL LEVELS IN CARBON (TONNES 
PER HECTARE) SIX MONTHS POST TILL AT 60-80 CM DEPTH - 
Test:LSD Fisher Alfa=0.05 DMS=12.34004 
Error: 48.1154 gl: 12 
Treatments Median n E.E. 
ORG B 7.05 3 4.00 A 
BD B 2.31 3 4.00 A B 
BD A 0.61 3 4.00 A B C 
ORG A -2.36 3 4.00 A B C 
Conv B -8.82 3 4.00 B C 
Conv A -12.55 3 4.00 C 
Medians that share the same letters are not statistically significant (p > 0.05) 
SOURCE: RAW DATA, TFST TEAM 
SEPT, 2014 
P VALUE = NOT STATISCALLY SIGNIFICANT
Sharper focus on Conventional A, 
where 13.88 tonnes (median) per 
hectare of carbon were 
introduced post tillage at a depth 
of 60-80 cm
Now focus on Conventional A 
after 9 months of growing cassava 
– 
Conventional A lost 12.55 
(median) of the additional 13.88 
tonnes C per hectare. It spent 
most of its inheritance.
Let’s focus now on Organic B, where 
approximately 8 tonnes of C were 
introduced post initial tilling, and 
approximately 7 additional tonnes 
were added over the next nine 
months. It earned a nice return on its 
inheritance!
And in every case the Biodynamic 
or Organic treatments maintained 
their carbon inheritance better 
than the conventional practices.
As a result, an “aggregate” look at 
the test lots, adding up the carbon 
at all depths, shows how the 
organic and biodynamic fields were 
far better at retaining their 
“inheritance,” in contrast to the 
conventional lots’ more profligate 
spending.
Spending the carbon inheritance 
treatment 
Mg/ha of C 
added after 
tilling 
Mg/ha of C that was 
able to retain 
Mg/ha of C 
lost 
% Mg/ha of C 
lost 
% Mg/ha of C 
retain 
Conventional 43,09 5,56 37,53 87,09% 12,91% 
Biodynamic 58,21 24,54 33,67 57,84% 42,16% 
Organic 37,00 15,54 21,46 57,99% 42,01%
These data do not achieve 
statistical significance with 95% 
confidence. But they paint a 
picture of what is happening real 
time in a field that is transitioning 
from being overgrazed for 65+ 
years to productive farming. This 
transitional moment is of critical 
importance.
Join us! 
www.thecarbonunderground.org
Tom Newmark - Field Trials in Costa Rica

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Tom Newmark - Field Trials in Costa Rica

  • 1.
  • 2. This is where my journey started, at Rodale’s FST in the Lehigh Valley
  • 3. 40% of the world’s population and 55% of children under the age of 5 live in the tropics. We need to demonstrate that regenerative agricultural practices work in the tropics.
  • 4. The Carbon Underground Tropical Farming Systems Trial Trial Design
  • 5. Same cassava starter cuttings, same farmers, same sun, same starter soil, same water
  • 6. Then a drought happened….
  • 9. Marked Failure to Germinate in Conventional Fields 2500 2000 1500 1000 500 0 BD.A BD.B OR.A OR.B CV.A CV.B Average number plants per treatment group Average number per treatment group that did not germinate
  • 10. 40.0000 35.0000 30.0000 25.0000 20.0000 15.0000 10.0000 5.0000 0.0000 Average % of plants that did not germinate per treatment group BD.A BD.B OR.A OR.B CV.A CV.B Average % did not germinate per treatment replication set
  • 11. We then replicated germination conditions in a more controlled environment, taking soil from each of the six farming systems and replicating the drought conditions by planting under greenhouse plastic.
  • 12. We found that the germination results under greenhouse plastic were the same as in the field. Here are two photos to show the differences between regenerative organic and conventional treatments.
  • 13. On the left, conventional. On the right, regenerative organic.
  • 14. Seeking explanations for the organic success vs. the conventional failure
  • 15. Bacterial Dynamics 14000 12000 10000 8000 6000 4000 2000 0 OR A OR B BD A BD B CV A CV B Average Bacteria Biomass (ug/g) Feb. 2014 Average Bacteria Biomass (ug/g) Apr. 2014
  • 16. Protozoan Dynamics 100000 90000 80000 70000 60000 50000 40000 30000 20000 10000 0 OR A OR B BD A BD B CV A CV B Average Protozan Population Feb. 2014 Average Protozan Population Apr. 2014
  • 17. Could there be a deeper cause? Recall that soil organic carbon can retain up to 40X its weight in water….
  • 18. So we dug down for information. More precisely, we took 810 soil samples down to 80 cm, with 135 samples per farming system
  • 19. And a note on determining carbon sequestration: we use the The Earth Partners’ soil carbon methodology, approved as a Verified Carbon Standard
  • 20. And now we have 9 months of data. Remember: the organic farming systems all used compost tea, compost mulch (applied at a rate of approximately 7 tonnes per hectare, of which approximately 2 tonnes is organic C), and only had an initial tilling. The conventional systems used herbicides, pesticides, and had two tillings.
  • 21. As you’ll see, after the initial tilling, where a decades-old weed mat was ploughed under, we introduced a significant amount of “labile” or unstable carbon over baseline.
  • 22. Let’s look at differing soil depths to see if we can find out what’s happening with soil organic carbon
  • 23. COA COB BDA BDB ORA ORB TREATMENT 0-10 kg of C per m² Pre-till 0-10 kg of C per m² Post-till 0-10 kg of C per m² 12 de agosto 78.50 67.29 56.07 44.85 33.63 kg of C per m² 43.77 44.67 42.61 46.54 41.23 48.53 Change in Kg of Carbon per m² at 0-10 cm SOURCE: RAW DATA, TFST TEAM SEPT, 2014 P VALUE = NOT STATISCALLY SIGNIFICANT
  • 24. COA COB BDA BDB ORA ORB TREATMENT 10-20kg of C per m² Pre-till 10-20 kg of C per Post-till 10-20 kg of C per m² 12 de agosto 57.70 49.80 41.90 34.00 26.10 kg of C per m² 32.57 32.72 36.42 36.95 33.57 36.28 Change in Kg of Carbon per m² at 10-20 cm SOURCE: RAW DATA, TFST TEAM SEPT, 2014 P VALUE = NOT STATISCALLY SIGNIFICANT
  • 25. COA COB BDA BDB ORA ORB TREATMENT 20-40 kg of C per m² Pre-till 20-40 kg of C per m² Post-till 20-40 kg of C per m² 12 de agosto 68.26 59.09 49.91 40.73 31.55 kg of carbon per m² 47.83 47.42 48.84 47.42 44.75 50.12 Change in Kg of Carbon per m² at 20-40 cm SOURCE: RAW DATA, TFST TEAM SEPT, 2014 P VALUE = NOT STATISCALLY SIGNIFICANT
  • 26. COA COB BDA BDB ORA ORB TREATMENT 40-60 kg of C per m² Pre-till 40-60 kg of C per m² Post-till 40-60 kg of C per m² 12 de agosto 54.60 45.40 36.21 27.02 17.83 kg of carbon per m² 28.16 30.44 35.13 33.63 38.52 41.13 Change in Kg of Carbon per m² at 40-60 cm SOURCE: RAW DATA, TFST TEAM SEPT, 2014 P VALUE = NOT STATISCALLY SIGNIFICANT
  • 27. COA COB BDA BDB ORA ORB TREATMENT 60-80 kg of C per m² Pre-till 60-80 kg of C per m² Post-till 60-80 kg of C per m² 12 de agosto 50.45 39.40 28.35 17.30 6.25 kg of carbon per m² 25.90 23.16 25.64 27.15 28.08 38.12 Change in Kg of Carbon per m² at 60-80 cm SOURCE: RAW DATA, TFST TEAM SEPT, 2014 P VALUE = NOT STATISCALLY SIGNIFICANT
  • 28. So let’s focus on the trends at the 60-80 cm depth
  • 29. MEDIAN LABILE CARBON ABOVE BASELINE AFTER THE TILLAGE AT A 60-80 CM DEPTH Test:LSD Fisher Alfa=0.05 DMS=11.51007 Error: 41.8608 gl: 12 Treatment Median n E.E. Conv A 13.88 3 3.74 A Conv B 9.85 3 3.74 A B ORG B 8.34 3 3.74 A B ORG A 4.37 3 3.74 A B BD B 1.74 3 3.74 B BD A 0.65 3 3.74 B Medians that share the same letter are not statistically significant (p > 0.05) SOURCE: RAW DATA, TFST TEAM SEPT, 2014 P VALUE = NOT STATISCALLY SIGNIFICANT
  • 30. MEDIAN CHANGE COMPARED TO POST TILL LEVELS IN CARBON (TONNES PER HECTARE) SIX MONTHS POST TILL AT 60-80 CM DEPTH - Test:LSD Fisher Alfa=0.05 DMS=12.34004 Error: 48.1154 gl: 12 Treatments Median n E.E. ORG B 7.05 3 4.00 A BD B 2.31 3 4.00 A B BD A 0.61 3 4.00 A B C ORG A -2.36 3 4.00 A B C Conv B -8.82 3 4.00 B C Conv A -12.55 3 4.00 C Medians that share the same letters are not statistically significant (p > 0.05) SOURCE: RAW DATA, TFST TEAM SEPT, 2014 P VALUE = NOT STATISCALLY SIGNIFICANT
  • 31. Sharper focus on Conventional A, where 13.88 tonnes (median) per hectare of carbon were introduced post tillage at a depth of 60-80 cm
  • 32. Now focus on Conventional A after 9 months of growing cassava – Conventional A lost 12.55 (median) of the additional 13.88 tonnes C per hectare. It spent most of its inheritance.
  • 33. Let’s focus now on Organic B, where approximately 8 tonnes of C were introduced post initial tilling, and approximately 7 additional tonnes were added over the next nine months. It earned a nice return on its inheritance!
  • 34. And in every case the Biodynamic or Organic treatments maintained their carbon inheritance better than the conventional practices.
  • 35. As a result, an “aggregate” look at the test lots, adding up the carbon at all depths, shows how the organic and biodynamic fields were far better at retaining their “inheritance,” in contrast to the conventional lots’ more profligate spending.
  • 36. Spending the carbon inheritance treatment Mg/ha of C added after tilling Mg/ha of C that was able to retain Mg/ha of C lost % Mg/ha of C lost % Mg/ha of C retain Conventional 43,09 5,56 37,53 87,09% 12,91% Biodynamic 58,21 24,54 33,67 57,84% 42,16% Organic 37,00 15,54 21,46 57,99% 42,01%
  • 37. These data do not achieve statistical significance with 95% confidence. But they paint a picture of what is happening real time in a field that is transitioning from being overgrazed for 65+ years to productive farming. This transitional moment is of critical importance.