Slides to accompany video: http://youtu.be/M-HNHLPpODs This presentation provides a brief introduction to the cre-lox system of tissue-specific genome remodeling, along with the tools in MGI (www.informatics.jax.org) for characterization of both floxed alleles and cre recombinase transgenes.

1. Module 2.
cre-lox and cre
recombinases in MGI

2. The cre-Lox system
1. mouse with a targeted
“floxed” allele
2. mouse with a cre
recombinase enzyme
expressed under the
control of a transgenic
promoter
 Breed together to
generate a line that
carries both
 Tissue specific deletion of
floxed allele in tissues
where cre recombinase is
expressed

3. Targeted conditional and
conditional-ready alleles

4. Summary of Brca1 alleles: use
category to identify attributes

5. Locating information about a
targeted allele
Reference

6. cre recombinase transgenes:
by driver or by tissue

8. cre recombinases with activity
examined in bone marrow

10. Recombinase Activity table

11. Systems level recombinase
activity detail

12. Summary
MGI allows users to discover of conditionally
targeted alleles and appropriate
recombinase
Transgene recombinase alleles are
characterized across the whole mouse with
data presented from multiple sources
contact mgi-help@jax.org with any questions